Phosphinothricin resistance

Author: vytg

August undefined, 2024

Webresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the WebFeb 18, 2024 · Two elite genotypes of P. tremula viz. Pt and f2 were transformed with BAR gene for development of phosphinothricin resistance. All the six transgenic lines …

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WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the … WebPhosphinothricin C5H11NO4P- CID 25244585 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities ... raymond bangladesh

Use of bar as a selectable marker gene and for the production of ...

WebResistance plasmids include pGreen II 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos- bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ … WebApr 11, 2024 · ----- Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20-203-01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl-(methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1.PPT is a structural analog of glutamate that binds to glutamine … raymond banfield

Genetic Engineered Resistance against Herbicide: Current status …

(PDF) The use of phosphinothricin resistance as ... - ResearchGate

WebInsertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower expression and commensurate reduction in PAT activity is a key factor in the low level of injury observed in the WideStrike(®) cotton and relatively high level of resistance observed in LibertyLink(®) … WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine Authors: Rahma Jardak Centre of Biotechnology of Borj-Cedria, Tunisia Belkacem Ould Bouamama... simplicity canisterWebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine R. J ARDAK -J AMOUSSI 1) , B. B OUAMAMA 1) , A. M LIKI 1) , A. G HORBEL 1) and G. M. R EUSTLE 2) simplicity car care 40 midwest

"WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the assimilation of ammonia accumulated as a result of photorespiration and nitrate reduction. " - Phosphinothricin resistance

Phosphinothricin resistance

Phosphinothricin Acetyltransferase - an overview

WebBialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as … WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by …

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WebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene ( bar) as a selectable marker was established, and two short peptide-encoding genes ( ant 1 and ant 2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. WebPhosphinothricin (PPT) is a potent inhibitor of glutamine synthetase in plants and is used as a non-selective herbicide. The bar gene which confers resistance in Streptomyces hygroscopicus to bialaphos, a tripeptide containing PPT, encodes a phosphinothricin acetyltransferase (PAT) (see accompanying paper).

WebNov 25, 2014 · Resistance against phosphinothricin: L-phosphinothricin (PPT) is a naturally occurring amino acid with herbicidal activity and it is active component of herbicide "Basta". PPT is a potent inhibitor of glutamine synthase and thus causes rapid increase in ammonia concentration in plants and this leads to death of plant cell. Web168 protocolsare often genotypespeciﬁc (Widholm, 1995; Somers et al., 2003). Increased soybean transforma-tion efﬁciency, may be achieved by further optimizing

WebBialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops1–4. WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

WebApr 11, 2024 · insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate …

WebThe present invention relates to specific herbicide combinations comprising (i) L-glufosinate and/or salts thereof and (ii) carfentrazone-ethyl in specific ratios, and to compositions comprising said herbicide combinations in these ratios. The present invention further relates to a method of producing said specific herbicide combinations and compositions … simplicity car care edmonton south eastWebNov 6, 2006 · Selection of phosphinothricin-resistant seedlings. The rapid selection method was also applied to a mixture of wild-type and phosphinothricin-resistant seedlings … raymond banks facebookWebGmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) as selection marker for the transgenic line. simplicity car care country hillsWebWild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant to PPT, but it was demonstrated that resistance is due to lack of PPT transport into the cells, which strongly suggests that PPT and l-methionine-S-sulfoximine enter the cells through different systems. Wild-type strain 21gr of the green alga Chlamydomonas reinhardtii was resistant … raymond bandy md raymond bangladesh websiteWebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … simplicity cape patterns for womenWebOct 23, 2015 · The R295K mutation has also never been previously reported as an important residue for PPT resistance. Furthermore, the mutant gene has been transformed into … simplicity canister vacuum reviews